Open-access databases such as the European Nucleotide Archive (ENA) contain more than 2.4 million bacterial genomes, and this number continues to grow rapidly. Until now, searching these vast ...
CRISPR guide RNA sequences deriving exactly from natural sequences may not perform optimally in every application. Here we implement and evaluate algorithms for designing maximally fit, artificial ...
Multiple sequence alignment (MSA) lies at the heart of comparative genomics, structural biology and evolutionary inference. By arranging three or more nucleotide or amino acid sequences in a matrix, ...