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纯*干货分享,细胞爬片详细操作步骤,收藏版本不要下次找不到我 免疫荧光细胞爬片精简操作 注意事项一、实验步骤1:制片贴壁细胞:孔板预置玻片,细胞贴壁后弃液,PBS洗1次;悬浮细胞:细胞滴于载玻片晾干,PBS洗1次。2:固定:4%多聚甲醛室温10~20min(或4℃过夜),轻柔PBS漂洗1次。3:透膜:0.1%Triton X-100室温通透20min,PBS洗3次。4:封闭:3%BS
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科研陪跑郑州大迈
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